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epifluorescence microscope eclipse 50i  (Nikon)


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    Structured Review

    Nikon epifluorescence microscope eclipse 50i
    Epifluorescence Microscope Eclipse 50i, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+50i+epifluorescence+microscope/pm40498268-82-35-39
    Average 90 stars, based on 1 article reviews
    epifluorescence microscope eclipse 50i - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Targeting metastasis-initiating cancer stem cells in gastric cancer with leukaemia inhibitory factor.
    Article Snippet: Immunofluorescence on tissue sections of paraffin-embedded collagen spheres followed the same protocol apart from an antigen demasking step carried out in 96 °C-hot pH 6 citrate buffer for 1 h. Primary antibodies used were mouse anti-human CD44v3 (R&D systems clone 3G5, Bio-Techne, Noyal Chatillon sur Seiche, France), rabbit anti-ZEB1 (Bethyl Laboratories, Montgomery, USA), rabbit anti-SNAI1 (H-130) (Santa Cruz Biotechnology, Bergheimer, Germany), mouse anti-TAZ (BD Biosciences, Grenoble, France); all at 1:100 for 1h30, and secondary fluorophore-labelled antibodies were Alexa Fluor® 488 goat anti-rabbit, Alexa Fluor® 594 donkey anti-mouse (1:200), Alexa Fluor® 647- Phalloidin (1:400), Alexa Fluor® 405 donkey anti-rabbit (1:250), combined with 4’-6-diamino-phenyl-indol (DAPI, 50mg/mL) or DiTOTM-3 (5 μM) (AAT Bioquest), for 1 h (all from Thermo Fisher). .. Images were taken using an Eclipse 50i epifluorescence microscope (Nikon, Champigny sur Marne, France) with NIS-BR acquisition software and a ×40 (numerical aperture, 1.3) oil immersion objective. .. ZEB1 and SNAIL mean nuclear intensity (mean Gray-value) or integrated density (mean Gray-value related to nuclear size variation for AGS) measurement was carried out using ImageJ 1.53f51 software [4, 10, 11, 43].

    Article Title: Bleomycin-induced chromosomal aberrations in Epstein-Barr virus-transformed human lymphoblastoid cells.
    Article Snippet: We have evaluated the induction of complete (i.e., without open ends) and incomplete (i.e., with non-rejoined or open ends) chromosomal aberrations by the radiomimetic antibiotic bleomycin (BLM) in human lymphoblastoid cells immortalized with the Epstein-Barr virus (EBV).. An EBV-induced lymphoblastoid cell line (T-37) was exposed to BLM (10–200 μg/mL) for 2 h at 37oC, and chromosomal aberrations were analyzed 24 h after treatment, using PNA-FISH with pan-telomeric and pan-centromeric probes.. Both complete (multicentrics, rings, compound acentric fragments, and interstitial deletions) and incomplete (incomplete chromosomes or IC, and terminal acentric fragments or TAF) chromosomal aberrations increased significantly in BLM-exposed cells, although the concentration-response relationship was non-linear.

    Article Title: First isolation of Campylobacter vicugnae sp. nov. in humans suffering from gastroenteritis.
    Article Snippet: .. Epifluorescence imaging was performed using Eclipse 50i epifluorescence microscope (Nikon, Champigny sur Marne, France) equipped with the DP23-M camera and the CellSens Standard software (Olympus, Tokyo, Japan), Nikon 20× objective. ..

    Article Title: Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of Drosophila melanogaster
    Article Snippet: .. Spermatids of fresh testes were analyzed by a phase contrast objective of a Nikon Eclipse 50i epifluorescence microscope to evaluate defects in cytokinesis. ..

    Article Title: Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of Drosophila melanogaster
    Article Snippet: .. Fluorescent 16-cell cysts and spermatids were observed with a Nikon Eclipse 50i epifluorescence microscope equipped with a CCD camera. .. Images were acquired with NIS-Elements software, provided by Nikon, and processed using Adobe Photoshop (Adobe Systems, Mountain View, CA, USA) and ImageJ software ( http://rsbweb.nih.gov/ij/ accessed on 1 March 2022).

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon).

    Article Title: Revision and analysis of the chromosome variability in the speciose genus Akodon (Rodentia, Sigmodontinae), including new data from Argentina.
    Article Snippet: .. Fluorescence microscopy was performed on a Nikon Eclipse 50i epifluorescence microscope equipped with an HBO 100 mercury lamp, a Nikon high-resolution digital color camera (DSRi-U3), and filters for DAPI and Cy3 (Chroma Technology Corp., Rockingham, VT, USA). ..

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n=8 per coverslip) were taken at a 40x magnification (0.085μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. A custom made MATLAB script (The Mathworks, Natick, MA, USA) was used to set a fluorescence intensity threshold for each staining.

    Software:

    Article Title: Targeting metastasis-initiating cancer stem cells in gastric cancer with leukaemia inhibitory factor.
    Article Snippet: Immunofluorescence on tissue sections of paraffin-embedded collagen spheres followed the same protocol apart from an antigen demasking step carried out in 96 °C-hot pH 6 citrate buffer for 1 h. Primary antibodies used were mouse anti-human CD44v3 (R&D systems clone 3G5, Bio-Techne, Noyal Chatillon sur Seiche, France), rabbit anti-ZEB1 (Bethyl Laboratories, Montgomery, USA), rabbit anti-SNAI1 (H-130) (Santa Cruz Biotechnology, Bergheimer, Germany), mouse anti-TAZ (BD Biosciences, Grenoble, France); all at 1:100 for 1h30, and secondary fluorophore-labelled antibodies were Alexa Fluor® 488 goat anti-rabbit, Alexa Fluor® 594 donkey anti-mouse (1:200), Alexa Fluor® 647- Phalloidin (1:400), Alexa Fluor® 405 donkey anti-rabbit (1:250), combined with 4’-6-diamino-phenyl-indol (DAPI, 50mg/mL) or DiTOTM-3 (5 μM) (AAT Bioquest), for 1 h (all from Thermo Fisher). .. Images were taken using an Eclipse 50i epifluorescence microscope (Nikon, Champigny sur Marne, France) with NIS-BR acquisition software and a ×40 (numerical aperture, 1.3) oil immersion objective. .. ZEB1 and SNAIL mean nuclear intensity (mean Gray-value) or integrated density (mean Gray-value related to nuclear size variation for AGS) measurement was carried out using ImageJ 1.53f51 software [4, 10, 11, 43].

    Article Title: First isolation of Campylobacter vicugnae sp. nov. in humans suffering from gastroenteritis.
    Article Snippet: .. Epifluorescence imaging was performed using Eclipse 50i epifluorescence microscope (Nikon, Champigny sur Marne, France) equipped with the DP23-M camera and the CellSens Standard software (Olympus, Tokyo, Japan), Nikon 20× objective. ..

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon).

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n=8 per coverslip) were taken at a 40x magnification (0.085μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. A custom made MATLAB script (The Mathworks, Natick, MA, USA) was used to set a fluorescence intensity threshold for each staining.

    Fluorescence:

    Article Title: Bleomycin-induced chromosomal aberrations in Epstein-Barr virus-transformed human lymphoblastoid cells.
    Article Snippet: We have evaluated the induction of complete (i.e., without open ends) and incomplete (i.e., with non-rejoined or open ends) chromosomal aberrations by the radiomimetic antibiotic bleomycin (BLM) in human lymphoblastoid cells immortalized with the Epstein-Barr virus (EBV).. An EBV-induced lymphoblastoid cell line (T-37) was exposed to BLM (10–200 μg/mL) for 2 h at 37oC, and chromosomal aberrations were analyzed 24 h after treatment, using PNA-FISH with pan-telomeric and pan-centromeric probes.. Both complete (multicentrics, rings, compound acentric fragments, and interstitial deletions) and incomplete (incomplete chromosomes or IC, and terminal acentric fragments or TAF) chromosomal aberrations increased significantly in BLM-exposed cells, although the concentration-response relationship was non-linear.

    Article Title: Revision and analysis of the chromosome variability in the speciose genus Akodon (Rodentia, Sigmodontinae), including new data from Argentina.
    Article Snippet: .. Fluorescence microscopy was performed on a Nikon Eclipse 50i epifluorescence microscope equipped with an HBO 100 mercury lamp, a Nikon high-resolution digital color camera (DSRi-U3), and filters for DAPI and Cy3 (Chroma Technology Corp., Rockingham, VT, USA). ..

    Imaging:

    Article Title: First isolation of Campylobacter vicugnae sp. nov. in humans suffering from gastroenteritis.
    Article Snippet: .. Epifluorescence imaging was performed using Eclipse 50i epifluorescence microscope (Nikon, Champigny sur Marne, France) equipped with the DP23-M camera and the CellSens Standard software (Olympus, Tokyo, Japan), Nikon 20× objective. ..

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 × magnification (0.085 μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon).

    Article Title: Neuroprotective role of lactate in a human in vitro model of the ischemic penumbra
    Article Snippet: .. Epifluorescent pictures (n=8 per coverslip) were taken at a 40x magnification (0.085μm/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon). .. A custom made MATLAB script (The Mathworks, Natick, MA, USA) was used to set a fluorescence intensity threshold for each staining.



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